Competent Cells
INTACT GENOMICS INC igMax™ DH10B ElectroCompetent Cells 6x100µl
Intact Genomics igMax™ DH10B derivative electrocompetent cells are suitable for demanding cloning situations such as synthetic bio-applications, BAC cloning, assembling large multi-DNA fragments, or cloning difficult targets requiring the greatest number of transformants possible. Utilizing proprietary manufacturing methods, these cells allow for effective transformation of all large DNA molecules (≥10kb up to 350kb)!Product Include and Storage:igMax™ DH10B Electrocompetent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4 ºC
Electron Microscopy Sciences ProPlate™ 96 Square FlexPlate, Adhesive 1 Side, 7.25x7.25mm Black
Patented ProPlate technology for high throughput robotic processing of microarrays. Grace BioLabs developed bottomless SBS compliant microtiter plate superstructures for use with robotic processors. Features guaranteed flat SBS compliant microtiter plate format which enables robotic processing of assays. It is compatible with glass or plastic substrates and has a water-tight seal between superstructure and substrate. 7.25x7.25mm, Black Polystyrene Frame, Adhesive One Slide - Bottomless In a removable well format.
Bioss E. coli
E coli Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Gold Biotechnology Inc LBA4404 Chemically Competent Agrobacterium 5x50µL
GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
INTACT GENOMICS INC GV3101 Agrobacterium Chemically Competent Cells 18x50µl
Intact Genomics GV3101 Agrobacterium Chemically Competent Cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potatoes, and monocots like corn.Product Includes and Storage:GV3101 Agrobacterium Chemically Competent Cells: -80 ºCpCAMBIA1391z control DNA: -20 ºCAgro Recovery medium: 4 ºC
INTACT GENOMICS INC HB101 Chemically Competent Cells 6x100µl
Intact Genomics (ig) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening.Reagents Needed for One Reaction:ig HB101 chemically competent cells: 50 µlDNA (or pUC19 Control, 10 pg/µl): 1 µlRecovery medium: 1 ml
BIOSEARCH TECHNOLOGIES INC (Lucigen) Endura Chemically Competent Cells, 12 Rxns (DUOs)Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Lucigens Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
Sigma Aldrich Fine Chemicals Biosciences BL21DE3 Electrocompetent C
The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1 000 fold relative to other preparations of BL21 cells which is essential for construction of complex expression libraries.Genotypef - ompT hsdSB (rB- mB-) gal dcm (DE3)
Sigma Aldrich Fine Chemicals Biosciences CelLytcTM Y Cell Lyss Re500ML
CelLytic Y Cell Lysis Reagent is versatile phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.
Bio Rad Laboratories AG1-X4 ANIONEXCHANGERESIN 500G
AG 1-X4 Anion Exchange Resin, analytical grade, 100–200 mesh, chloride form, 500 g #1401341500 g, analytical grade anion exchange resin, chloride form, 4% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~1,400 MW limit
Zymo Research Corporation Mix & Go! Competent Cells- Zymo 10B (96 x 50 μl)
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/g of pUC19 DNA, which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 l/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 l/tube.
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni 10GF' ELITE Electrocompetent Cells, 12 Rxns (DUOs)Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies
Direct replacements for standard cloning strainsOptimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.Available in a range of high transformation efficienciesConvenient packaging options.Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.